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KMID : 0379120120400030132
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Korean Journal of Mycology
2012 Volume.40 No. 3 p.132 ~ p.135
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Identification of Orchid Mycorrhizal Fungi Isolated from Five Species of Terrestrial Orchids in Korea
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Youm Jae-Young
Han Han-Kyeol
Chung Jae-Min
Cho Yong-Chan
Lee Byung-Chun
Eom Ahn-Heum
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Abstract
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This study was performed to isolate and identify the orchid mycorrhizal fungi (OMF) from roots of five species of terrestrial orchids in Korea; Cymbidium goeringii, Spiranthes sinensis, Calanthe discolor, Bletilla striata, Pogonia minor. DNA was extracted from isolated OMF and ITS region was amplified using primers, ITS1-OF and ITS4-OF. Four species of OMF belong to Tulasnellaceae and Sebacinaceae were identified; Tulasnella calospora, Tulasnella irregularis, Tulasnella sp., Sebacina vermifera.
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KEYWORD
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Cymbidium goeringii, ITS, OMF, Orchid, Sebacinaceae, Tulasnellaceae
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